Emerging research suggests a compelling link between the ketogenic diet and alterations in DNA methylation patterns. This remarkable dietary approach, characterized by high fat intake and restricted carbohydrate consumption, may profoundly influence gene expression by modifying the epigenome. While the precise mechanisms remain under investigation,
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remedy with sodium bisulfite deaminates unmethylated cytosine to uracil, Whilst 5mC is check here not really afflicted. Subsequent PCR amplification will swap the uracils by thymines. Reads from NGS libraries designed in parallel with and without bisulfite remedy are mapped on to reference genomes as well as contrasting C/T positions among the libr