THE ULTIMATE GUIDE TO GENETIC METHYLATION TEST GARY BREAKA

remedy with sodium bisulfite deaminates unmethylated cytosine to uracil, Whilst 5mC is check here not really afflicted. Subsequent PCR amplification will swap the uracils by thymines. Reads from NGS libraries designed in parallel with and without bisulfite remedy are mapped on to reference genomes as well as contrasting C/T positions among the libr